Disposable Cell Concentration Chip
Key Features
- User-friendly disposable chip
- Centrifugation-free workflow
- 95% cell recovery
- 20x cell enrichment in 2 minutes
- Clean bench operation
- Automated enrichment process
Cellpuri™ is a disposable chip that concentrates cells without the centrifugation process. The chip enriches cells using rheological phenomenon inside the microchannels where cell suspension passes through to filter out waste medium. The chip is composed of an inlet in which the cell suspension is loaded, outlet where the cell-enriched suspension is collected and cell free medium (waste) is filled at waste area. The size of Cellpuri is 76 (w) x 26 (d) x 23 (h) mm where it can process 2 ~ 20mL of cell suspension at the speed of 1 mL/min. Furthermore, Cellpuri can concentrate cells with 7~15μm in size and enriches more than 20 times with respect to the original cell concentration.
What is Filterless Filter (FLF) Technology?
It is a microfluidic chip-based cell separation and concentration technology developed by Curiosis. Countless micrometer-sized channels inside the chip create a rheological flow that separates, concentrates, and removes particles of a specific size from the solution. Microchannels were strategically designed to isolate cells according to their size where larger cells are directed towards one side whereas smaller cells flow randomly on lateral axis that eventually compile on the other side. FLF technology can be applied to cell enrichment, white blood cell separation, and blood-plasma separation.
Cell enrichment by Cellpuri®
Cellpuri® efficiently separated HL-60 cells from medium without spinning-down the cells and more than 20-fold enrichment (121.2x10 cells/mL) was observed in HL-60 cells at an initial concentration of 4.9 x 10 cells/mL.
Reduced cell clumping with Cellpuri®
Adherent cells, including MCF7 cells, tend to form clumps during the cell passage. But as shown in above images, enrichment using Cellpuri® reduces cell clumping while centrifugation pellets the cells and thus increasing the clumping cells.
