isoHub
An automated microscope that can be combined with an isoCell or isoPick as part of either the Cloning Platform or scPicking Platform, respectively. Inclusion of the isoHub enhances workflows for both platforms, enabling quick and easy single-cell chamber selection when scanning through GRIDS, and seamless data transfer between devices.
isoHub
Easily Verify Monoclonality and Track Your Clones
After single cells are plated into GRID chambers they are imaged using the isoHub. Entire GRID chambers can be easily reviewed in the absence of optical edge effects typically associated with conventional culture plasticware. That way, you get absolute confidence in identifying chambers that contain a single cell. The isoHub also automates navigation through GRIDs and allows tracking of your selected clones, either using brightfield or fluorescent mode. What’s more, automated wireless data-transfer between the isoCell and isoHub guarantees a seamless and efficient workflow.
Performance and functions
- Automate all tedious pipetting steps
- Confidently document monoclonality with whole-chamber images
- Maintain exceptional cell viability
- Easily navigate the entire workflow with intuitive touchscreens
- Compatible with a wide range of reagents
isoHub Overview
SIMPLE SINGLE-CELL VERIFICATION
As GRIDS are made using only fluids, this eliminates the problem of optical edge effects that comes with the use of conventional plasticware. As a result, single cells are visible on the isoHub immediately in a single 10x objective field of view after plating, enabling quick and easy verification of singularity. Phase contrast microscopy with the isoHub makes visualisation and identification of single cells simple.
EASY-TO-NAVIGATE
The hand-held controller and automated stage make it easy to move between GRID chambers and select those containing single cells of interest. Eliminating the need for manual movements through sequential GRID chambers and manually noting chamber coordinates, this further streamlines your single-cell workflows. The isoHub automatically records the coordinates of selected chambers, saving time and reducing the chance of error.
FLUORESCENCE MICROSCOPY (OPTIONAL)
A compact, but highly efficient optional fluorescence module can be connected to the isoHub, allowing the screening of cells/colonies via fluorescence microscopy. With a 16-channel LED light source and simple on/off button operation, the addition of fluorescence to your single-cell workflow couldn't be easier.
SEAMLESS WIRELESS DATA TRANSFER
Wireless communication between paired isoHub and isoCell or isoPick instruments enables the automatic synchronisation of data, so you are always ready for the next step in the workflow.
IMAGE CAPTURE SYSTEM (OPTIONAL)
Do you need to record the morphology of your cells of interest, document single-cell confirmation, prove monoclonality or collect images of single-cell outgrowth? If so, an optional imaging system can be added to the isoHub to facilitate such requirements. Comprising of a high-resolution camera with PC, large touch screen, and our isoHub Image Capture App, you'll have everything you need to record visual information throughout your single-cell workflow. The system makes it easy to capture and store high-resolution photos, providing evidence of monoclonality during a cloning workflow for example.
Specifications
Details
