Cloning Platform
An automated single-cell cloning workflow, with easy verification of monoclonality in small volumes. It comprises the isoCell, a compact nanolitre fluid handling system, and the isoHub, a smart automated microscope for simple verification of single-cells and easy tracking of clonal cultures.
Cloning Platform
Single-Cell Cloning and Imaging
The Cloning Platform is a complete workflow solution for single-cell cloning. It offers automated handling of miniturised culture volumes as low as 200 nL and easy confirmation of monoclonality on day 0. Gentle handling with built-in programs for plating cells, feeding colonies, and harvesting clones maximises cloning efficiencies. With imaging and fluorescence options available, there's a configuration to suit your needs.
Performance and functions
- Select cells/colonies using fluorescence (optional)
- Low pressure of <0.5 psi makes cell handling incredibly gentle
- Minimises cell culture media usage with only 600 nL per chamber
- Up to 25x more clones per mL of culture medium compared to other methods
- Easily navigate the entire cloning workflow with intuitive touchscreens
- Identify single-cell chambers with a single press of a button
- Document monoclonality and single-cell outgrowth as desired via imaging (optional)
- Highest assurance of clonality, as single cells can easily be visualised immediately after plating on day 0 in a single field of view
Suitable for single-cell cloning of:
- hiPSCs
- ESCs
- HEK293
- CHO
- RPE1
Cloning Platform Parts
IsoHub - easily verify monoclonality and track your clones
After single cells are plated into GRID chambers they are imaged using the isoHub. Entire GRID chambers can be easily reviewed in the absence of optical edge effects typically associated with conventional culture plasticware.
IsoCell - much more than a single-cell dispenser
The isoCell instrument automates all liquid-handling. It dispenses cells, exchanges culture medium and harvests clonal cultures for downstream applications — without the need for tedious manual pipetting.
Image Capture System - document monoclonality with whole-chamber images
The Image Capture System lets you document monoclonality and clonal outgrowth in a single click using whole-chamber imaging. Simply select GRID chambers that contain a single cell, and the imaging system records and documents your selection with a whole-chamber image.
isoHub Fluorescence — 4 channels and 16 LED wavelengths to detect any fluorophore
The Cloning Platform features a CoolLED pE-4000 illumination system for fluorescence microscopy. Easily detect commonly used fluorescent molecules (GFP, RFP, DAPI, and more) thanks to the system’s 4 channels, 16 LED wavelengths, and a user-friendly control pad.
Cloning Platform Overview
OPTIONAL FLUORESCENCE MICROSCOPY
Fluorescence microscopy is available as an optional add-on with a 16-channel LED light source. The isoHub comes with brightfield and phase contrast microscopy with 4x, 10x and 20x phase contrast objectives as standard.
WIRELESS DATA TRANSFER
A wireless connection between the isoHub and isoCell allows both systems to automatically synchronise data, so it is ready when you need it for the next step in the Cloning Platform workflow.
COMPACT SYSTEM
The small footprint of the isoCell easily fits inside your cell culture hood with room to spare, while the isoHub can be used as a general cell culture microscope with standard microtitre plates and other lab plasticware.
MONOCLONALITY
GRIDs containing 256 chambers are made on 60 mm
dishes using only fluids and so do not suffer the edge
effects of conventional culture plasticware. As a
result, chambers with single cells are visible
immediately after plating, enabling easy verification
of monoclonality.
WORKFLOW AUTOMATION
The isoCell isolates, feeds and harvests your cells to achieve better consistency in your cloning experiment, while the isoHub moves between chambers of interest allowing easy tracking of monoclonal colonies. The integrated touch screen on both machines provides an intuitive step-by-step guide through the cloning workflow.
Specifications
Details
