FastPure Cell/Tissue Total RNA Isolation Kit V2

RC112-01

The FastPure Cell/Tissue Total RNA Isolation Kit V2 provides a rapid method for extracting total RNA from animal tissues and cells. The kit is based on silica gel membrane purification technology and does not require β-mercaptoethanol, phenol/chloroform, or any other toxic reagent during the extraction process. It takes only 6 min to extract high-quality RNA. The kit contains FastPure gDNA-Filter Columns III which can effectively remove impurities and gDNA. FastPure RNA Columns III can efficiently bind RNA with an optimized buffer to obtain high-purity total RNA. The isolated RNA has little gDNA residues and no proteins or other impurities contamination. It can be used for RT-PCR, Real-Time PCR, Microarrarys and other downstream experiments.

Features

Fast

It only requires 6 min at room temperature.

Safer

No need for β-mercaptoethanol and phenol or chloroform.

Minimal gDNA residue

No need for on-membrane digestion with DNase I, one-step gDNA contamination removal.

High RNA purity

High purity of extracted RNA, which is ready for use in demanding downstream experiments.

Widely applicable

Compatible with all kinds of cell and tissue samples.

Performance

Super Fast

Obtain one samples in just 6 min at room temperature.

High Quality

Vazyme #RC112 ('V' in the following figures) and commercially available related products (from suppliers A, B, C, D, E ,F and G respectively) were used to extract total RNA from the rat liver tissue (10 mg) and HEK 293 cell (1×106) samples according to the respective Instructions for Use. Figure A is the agarose gel electrophoretogram of the extracted RNA, while Figures B and C are the statistical charts of RNA concentration and purity results. As shown in the figures, RNA extracted by Vazyme #RC112 has good integrity, high yield and high purity.

Wide Sample Compatibility

Vazyme #RC112 was used for the RNA extraction and purification of different samples, and the final RNA product was detected by agarose gel electrophoresis. It shows that Vazyme #RC112 is well compatible in the extraction of RNA from different cell and animal tissue samples.

Here shows some data from various samples

Note: Typical yields can vary due to factors such as species, developmental stage, and growth conditions.

Source	Amount	Elution (µl)	RNA (µg)
Cell	HEK 293	2 × 10⁶	100	8 - 10
HeLa	1 × 10⁶	100	10
A549	3 × 10⁵	50	7
Hacat	1.8 × 10⁷	50	45 - 50
HepG2	1 × 10⁵	50	0.3 - 0.4
5 × 10⁴	30	7
Macrophage	5 × 10⁴	30	28
Animal Tissue	Rat Liver	10 mg	100	35 - 50
1 mg	50	3 - 4
Rat Brain	10 mg	100	3 - 5
Rat Kidney	10 mg	100	25 - 30
Rat Spleen	10 mg	100	30 - 40
1 mg	50	3 - 4
Rat Heart	10 mg	100	5 - 7
Rat Lung	10 mg	100	10 - 15
Rat Muscle	10 mg	100	3 - 5
Rat Fat	10 mg	100	2 - 4
1 mg	50	1 - 2
Rat Intestine	10 mg	50	40 - 50
Bacteria	Escherichia coli	1 × 10⁹	50	60 - 80
Staphylococcus aureus	50	15 - 25
Enterococcus	50	6 - 10

Source		Amount	Elution (µl)	RNA (µg)
Cell	HEK 293	2 × 10⁶	100	8 - 10
	HeLa	1 × 10⁶	100	10
	A549	3 × 10⁵	50	7
	Hacat	1.8 × 10⁷	50	45 - 50
	HepG2	1 × 10⁵	50	0.3 - 0.4
	HepG2	5 × 10⁴	30	7
	Macrophage	5 × 10⁴	30	28
Animal Tissue	Rat Liver	10 mg	100	35 - 50
	Rat Liver	1 mg	50	3 - 4
	Rat Brain	10 mg	100	3 - 5
	Rat Kidney	10 mg	100	25 - 30
	Rat Spleen	10 mg	100	30 - 40
	Rat Spleen	1 mg	50	3 - 4
	Rat Heart	10 mg	100	5 - 7
	Rat Lung	10 mg	100	10 - 15
	Rat Muscle	10 mg	100	3 - 5
	Rat Fat	10 mg	100	2 - 4
	Rat Fat	1 mg	50	1 - 2
	Rat Intestine	10 mg	50	40 - 50
Bacteria	Escherichia coli	1 × 10⁹	50	60 - 80
	Staphylococcus aureus		50	15 - 25
	Enterococcus		50	6 - 10

Components

Components	RC112-01 (50 rxns)
Buffer RL	35 ml
Buffer RW1	45 ml
Buffer RW2	20 ml
RNase-free ddH₂O	10 ml
FastPure gDNA-Filter Columns III (each in a 2 ml Collection Tube)	50
FastPure RNA Columns III (each in a 2 ml Collection Tube)	50
RNase-free Collection Tubes 1.5 ml	50

Components	RC112-01 (50 rxns)
Buffer RL	35 ml
Buffer RW1	45 ml
Buffer RW2	20 ml
RNase-free ddH₂O	10 ml
FastPure gDNA-Filter Columns III (each in a 2 ml Collection Tube)	50
FastPure RNA Columns III (each in a 2 ml Collection Tube)	50
RNase-free Collection Tubes 1.5 ml	50

Storage

Store at 15 ~ 25°C and transport at room temperature.

Downloads

RC112 Reagent for Nuceleic Acid Extraction

Manual_RC112 V24.2

Protocol_RH104-C1_V26.1