2 × Rapid Taq Plus Master Mix (Dye Plus)
2 × Rapid Taq Plus Master Mix (Dye Plus)
P223-02
Rapid Taq Plus DNA Polymerase is a new generation Taq DNA polymerase with a high amplification success rate. This product combines high blocking rate dual-species antibodies (binding different antigenic epitopes) and an optimized buffer system, demonstrating excellent success rate and specificity at an extension rate of 1 - 10 sec/kb. The pre-prepared 2 × Master Mix only requires the addition of primers and templates for PCR amplification, reducing pipetting operations and increasing detection throughput and result reproducibility. This product has robust amplification performance and high storage stability. It supports PCR amplification of genomic, plasmid, and λDNA templates up to 15 kb, and cDNA templates up to 10 kb. The unique protectant added to the product ensures that 2 × Master Mix does not freeze when stored at -20°C, allowing for convenient use. Additionally, this product contains tracking dyes, enabling direct electrophoresis analysis after the reaction. The amplification products are applicable for ClonExpress Ultra One Step Cloning Kit V3 (Vazyme #C117) and Ultra-Universal TOPO Cloning Kit (Vazyme #C603).
Features
Rapid amplification
15 sec/kb; extension time up to 1 sec/kb (<2kb fragments)
Hot start
Dual-blocking antibody enables superior specificity and sensitivity
Broad compatibility
Works with 24%-79% GC systems and 7-plex amplification
Convenience
Store at -20°C without freezing, ready to use and include tracking dye
Experimental Cases
Ultra-fast Amplification
Vazyme #P223, Supplier A, and Supplier B were used to amplify the 2 kb fragment according to the reaction programs recommended in their respective manuals. P223 completed the amplification in just 30 minutes, significantly reducing the time required.
Broad Compatibility
Vazyme #P223 and Suppliers A-E were used to test the compatilbility of GC-poor/rich systems and different types of templates. The results show that P223 is compatible with systems having a GC content range of 24% to 79%, animal and plant lysates, and bacterial plaques/cultures. lts amplification success rate and specificity are superior to those of Suppliers A-E.
Components
Storage
Store at -30 ~ -15°C and ship at ≤0°C.
