isoPick
An advanced single-cell picking system designed to make precise, gentle, and reproducible cell isolation accessible to every lab. Built on iotaSciences’ patented isoCell microfluidic technology, isoPick streamlines single-cell selection and transfer eliminating manual pipetting, reducing contamination risks, and ensuring cell integrity throughout the workflow.
isoPick
IsoPick: Isolate and Pick Single Cells
The isoPick automates all fluid handling for the dispensing, isolation and transfer of single cells. Using our GRID technology, this instrument gently dispenses cells into nanolitre chambers for easy visualisation without optical edge effects, then flexibly transfers selected single cells into a range of different formats. Compatible with a range of downstream applications, the isoPick is a flexible instrument that enables the isolation of the single cells that matter to you. Optional sample cooling and ultra-gentle handling of sensitive cells ensures maximum cell viability during the isolation and transfer process.
Feature Highlights:
- Reproducible and consistent results with powerful automation
- Highest assurance of single cell isolation and transfer – up to 100%
- Low pressure of <0.5 psi ensures cell handling is incredibly gentle
- Maintains exceptional cell viability
- Easily navigate the entire single-cell isolation workflow with an intuitive touchscreen
- Flexible cell transfer into different formats - 96-well TC plates, 96-well PCR plates, 8-tube PCR strips, or LC-MS vials
- Optional sample cooling
Suitable for downstream applications such as:
- Single-cell cloning
- Single-cell genomics
- Single-cell lipidomics
- Microalgae isolation
- And many more – get in touch to discuss your requirements
isoPick Overview
SINGLE-CELL VERIFICATION
The isoPick plates single cells into GRID chambers, which do not suffer the optical edge effects of conventional plasticware. As a result, your single cells are visible immediately in a single field of view after plating, enabling easy verification using any inverted microscope with 4x/10x objectives.
WORKFLOW AUTOMATION
The isoPick gently isolates your cells with a low pressure of <0.5 psi, ensuring extremely low shear stress to maintain cell integrity and viability. The automated single-cell isolation workflow enables high consistency within your experiments via the integrated nanolitre fluid handling system. This eliminates many of the manual pipetting steps that can often be sources of experimental variation at low volumes.
FLEXIBLE APPLICATIONS
The isoPick is designed with flexibility in mind, not limiting users to a single collection format or specific experimental workflow. Cells can be deposited into several popular formats suitable for a variety of downstream single-cell applications. Whether you're planning on performing cloning, genomic analysis or mass spectrometry, the isoPick can be integrated as part of your experimental design.
INTEGRATED COOLING
Optional sample cooling is available for the entire picking workflow with the simple addition of cooling blocks for any application that requires this capability. The quick slide and lock fitting makes attachment easy and rapid cooling is achieved within minutes.
COMPACT SYSTEM
The small footprint of the isoPick easily fits inside any biosafety cabinet, with room to spare. The integrated touch screen provides an intuitive step-by-step guide through the picking workflow. If necessary, the isoPick can even be moved by a single person due to its low weight.
scPICKING PLATFORM
The isoHub makes identifying single cells easy and efficient and allows the optional addition of imaging and fluorescence capabilities. A wireless connection between the isoPick and isoHub enables both systems to automatically synchronise data for a more seamless picking workflow.
Efficient Single-Cell Isolation
The isoPick transfers verified single cells from ultra-low volume culture chambers (GRIDs) automatically into 96-well plates.
This approach provides a highly efficient and hands-off single-cell isolation process. The picking and transfer process is very gentle to cells and maintains highest cell viability throughout the entire workflow.
GRIDS - unprecedented optical clarity for your single cells
200-600
Nanolitres per chamber for efficient isolation and/or culturing single cells
120
Seconds for plating cells across 256 GRID chambers-automated & hands-free
256
Individual chambers on 60-mm polystyerene dishes
3.2
square millimetres per chamber
