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FlysisAmp Cells Lysis Kit

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    FlysisAmp Cells Lysis Kit

    CL101-01

    The FlysisAmp Cells Lysis Kit is a fast, efficient kit for harvesting cellular RNA and is compatible with 10 - 106 cultured cells (adherent or suspension cells). Genomic DNA elimination and RNA acquisition can be completed directly in the cell culture plate, and the whole process only takes 7 min. This method does not require the steps of heating, washing, tube transfer, and centrifugation  in  traditional  extraction  methods,  which  improves  efficiency  while  also  reducing  losses  during sample purification. This kit is compatible with a wide range of cell types and cell quantities, and can also solve the problem of unsatisfactory extraction results with low cell numbers in traditional extraction methods. This kit can be used for high-throughput (96-/384-well cell culture plate) acquisition of cellular RNA and for various downstream experiments such as RT-PCR and RT-qPCR.


    Features

    Fast and simple operation

    Takes 2 steps 7 - 9 min to remove gDNA and obtain cellular RNA.

    Wide sample compatibility

    Compatible with 10 - 106 adherent/suspension cells.

    High throughput

    Compatible with different cell culture plates (384/96/24/12/6), RNA acquisition can be done directly in well plates.


    Components

    Components CL101-01 100 rxns (50 µl/rxn)
    FlysisAmp Cells Lysis Buffer* 5 ml
    DNase I 200 µl
    Enhancer Solution 200 µl
    FlysisAmp Cells Stop Buffer 500 µl

    * FlysisAmp Cells Lysis Buffer can be stored stably for 1 year at 2 ~ 8°C after thawing.


    Storage

    Store at -30 ~ -15°C and transport at ≤0°C.


    Downloads

    Manual_CL101 V24.1