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BHMMP01S020 UltraScence Precut PVDF Membrane Sandwiches (0.2 μm, 7.3 x 8.3cm, 20 Sheets)

Size: 7.3 cm x 8.3 cm

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    UltraScence Precut PVDF Membrane Sandwiches

    BHMMP01S020

    For analyzing the small amounts of proteins (down to 10 pmoles), peptides or amino acids, polyvinylidene difluoride (PVDF) membranes are the most ideal item for tracing down these transferred small molecular weight materials after electroblotting. The UltraScence PVDF/Filter Paper Sandwiches, 0.2 μm, have excellent binding properties for western blotting, dot-blot assays, and other protein or nucleic acid methods such as protein sequencing.



    Specifications

    Specification Details
    Dimensions/Size 7.3 cm x 8.3 cm
    Material PVDF membrane
    Wettability Hydrophilic
    Thickness 140~150μm
    Pore Size 0.2 μm
    BSA Protein Binding Capacity ~300 μg/cm2


    Performance

    PVDF membrane
    Brand Catalog Lot number Protein Binding Capacity Ratio
    Bio-Helix MMP02-S020 17 2194 3007 77.3%
    PALL BSP0161 00749A 42.7%

    Table 1. The protein binding capacity of the PVDF membrane.

    The PVDF membranes were incubated in 0.5 mg/ml protein solution at room temperature for 24 hrs. Absorbance values were detected at 280 nm by the BCA assay, and the protein binding capacity can be calculated.

    * PALL is a registered trademark of PALL Corporation. The trademark holder is not affiliated with Bio-HeliX Co., Ltd. and does not recognize this product.


    Figure 2. The image of the PVDF membrane after wet transfer and semi-dry transfer of Tris-Glycine SDS-PAGE.


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